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目的:探讨血单个核细胞上PRLR mRNA的表达在桥本氏甲状腺炎患者中的诊断研究。方法:选2014年8月至2016年8月我院诊断桥本氏甲状腺炎患者和健康体检者各40例,分为观察组和对照组,两组均采用RQ-PCR定量检测PRLR mRNA,酶联免疫法测定IL-2,化学发光法测定血清中PRL、甲状腺球蛋白抗体(TGAb)和甲状腺过氧化物酶抗体(TPOAb)。结果:桥本甲状腺炎患者PRLR mRNA含量、PRL水平、TGAb、TPOAb、IL-2水平均显著高于正常组,两组比较差异有统计学意义(P<0.05)。结论:桥本氏甲状腺炎患者血单个核细胞上PRLR mRNA的表达增高,检测其含量、PRL水平、IL-2水平可作为诊断桥本氏甲状腺炎的一种手段,提高疾病诊断与治疗效果。
Objective: To investigate the diagnostic value of PRLR mRNA expression in blood mononuclear cells in Hashimoto’s thyroiditis patients. Methods: Forty Hashimoto’s thyroiditis patients and healthy volunteers were selected from August 2014 to August 2016 in our hospital. The patients were divided into observation group and control group. RQ-PCR was used to detect PRLR mRNA, IL-2 was measured by the method of chemiluminescence and serum PRL, thyroglobulin antibody (TGAb) and thyroid peroxidase antibody (TPOAb) were determined by chemiluminescence method. Results: The levels of PRLR mRNA, PRL, TGAb, TPOAb and IL-2 in patients with Hashimoto’s thyroiditis were significantly higher than those in the normal control group. There was significant difference between the two groups (P <0.05). Conclusion: The expression of PRLR mRNA on blood mononuclear cells in patients with Hashimoto’s thyroiditis is increased. The levels of PRLR, IL-2 and PRL can be used as a measure to diagnose Hashimoto’s thyroiditis and improve the diagnosis and treatment of Hashimoto’s thyroiditis.