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目的 探讨转化生长因子 α(TGF α)、表皮生长因子受体 (EGFR)反义寡脱氧核苷酸(ASODN)对人大肠癌细胞株HR8348的作用。 方法 将TGF α、EGFR反义寡核苷酸经脂质体包裹后转染人大肠癌细胞 (浓度均为 10 μmol/L)。采用四唑蓝比色法 (MTT法 )、细胞周期分析、裸鼠体内接种等方法测定瘤细胞体内外增殖的变化。结果 TGF α反义ODN、EGFR反义ODN均可明显抑制HR8348的增殖 (抑制率分别为 80 .0 %、82 .5 % ) ,并使裸鼠体内成瘤率下降 (均为2 0 .0 % ;对照组为 10 0 .0 % )。G0 /G1期细胞数明显增多 (分别为 6 3 .9%、6 7.1% ;对照组为31.1% ) ,S期细胞数相应减少 (分别为 35 .6 %、32 .6 % ;对照组为 5 3 .9% )。结论 TGF α反义ODN、EGFR反义ODN均能有效抑制大肠癌细胞的体内外生长增殖
Objective To investigate the effect of transforming growth factor α (TGF α) and epidermal growth factor receptor (EGFR) antisense oligodeoxynucleotide (ASODN) on human colorectal cancer cell line HR8348. Methods TGFα and EGFR antisense oligonucleotides were transfected into human colorectal cancer cells (10 μmol/L) by liposome encapsulation. The in vitro and in vivo proliferation of tumor cells was measured by tetrazolium blue colorimetric method (MTT method), cell cycle analysis, and inoculation in nude mice. Results TGFα antisense ODN and EGFR antisense ODN significantly inhibited the proliferation of HR8348 (80% inhibition and 82.5 % inhibition respectively), and decreased the tumorigenesis rate in nude mice (both 20%). %; the control group was 10 0.0%). The number of G0/G1 phase cells increased significantly (63.9%, 6 7.1%, respectively; the control group, 31.1%). The number of cells in S phase decreased accordingly (35.6% and 32.6%, respectively; the control group 5 3 .9%). Conclusion TGFα antisense ODN and EGFR antisense ODN can effectively inhibit the growth and proliferation of colorectal cancer cells in vivo and in vitro.