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目的 研究表没食子儿茶素没食子酸酯(EGCG)对急性胰腺炎相关性肺损伤的作用及机制.方法 用随机数表法将balb/c小鼠分为3组:空白对照组腹腔注射0.9％ NaCl(10 mL·kg-1);模型组腹腔注射雨蛙素50 μg·kg-1建立小鼠急性胰腺炎肺损伤模型,这2组均每小时注射1次,共7次;实验组在造模后1,3,6h,分别腹腔给予EGCG 25 mg·kg-1.于造模后24 h,用脱颈法处死小鼠.眼球取血,切取肺,取胰腺,进行病理学检查;用全自动生化分析仪测定血清中淀粉酶,以硫代巴比妥酸法检测丙二醛(MDA)水平,以酶联免疫吸附法(ELISA)测定肿瘤坏死因子(TNF-α)的含量,以免疫组化SP法检测肺组织核因子(NF-кB)水平,用苏木精-尹红(HE)染色法进行肺组织学观察及组织病理学评分.结果 实验组、模型组与对照组的血清淀粉酶分别为(3613.21±351.87),(4720.43±672.24),(895.41±107.18)U·L-1;这3组的MDA分别为(13.06±0.11),(15.49±0.40),(4.26±0.69) mmol·mL-;这3组的TNF-α分别为(59.83±14.74),(83.29 ±24.10),(24.76±10.24) pg· L-1;这3组的肺组织NF-KB表达分别为0.38±0.14,0.55±0.12,0.14±0.09;这3组的肺组织病理学评分分别为(3.47±1.20),(6.54±0.51),(0.21±0.07)分;模型组与对照组比较,以上指标差异均有统计学意义(均P＜0.05);实验组与模型组比较,以上指标差异均有统计学意义(均P ＜0.05).结论 EGCG可通过抑制NF-кB的表达和提高对氧自由基的清除,从而减轻实验小鼠胰腺炎症相关肺组织损伤.“,”Objective To study the effect of epigallocatechin-3-gallate(EGCG) on acute pancreatitis associated lung injury (APALI) in mice.Methods The balb/c mice were randomly divided into three groups:control group,model group and experimental group.APALI model mice were induced by intraperitoneal injection of crulein (50 μg · kg-1 body weight) in 0.9％ NaCl solution once per hour for 7 times.In control group,mice were only received the same volume of 0.9％ NaCl without caerulein.In experimental group,EGCG (25 mg· kg-1) was injected in abdomen at 1,3 and 6 h after the induction of APALI.Mice from each group were killed,then the correlated indexes were evaluated at 24 h after the induction of APALI.The levels of anylase,tumor necrosis factor (TNF-α),malondialdehyde (MDA) in serum and nuclear factor of kappa B (NF-кB) activity in lung tissue were determined.Hematoxylin-eosin (HE) staining was performed to evaluate the morphologic changes in animal lung.Results Levels of amylase in experimental group,model group and control group were (3613.21 ± 351.87),(4720.43 ± 672.24),(895.41 ± 107.18) U · L-1;MDA levels in the three groups were (13.06 ±0.11),(15.49 ±0.40),(4.26 ±0.69) mmol · mL-1;TNF-αt levels in the three groups were (59.83 ± 14.74),(83.29 ±24.10),(24.76 ± 10.24) pg · L-1;expression of NF-κB in the three groups were 0.38 ± 0.14,0.55 ± 0.12,0.14 ± 0.09;Histopathological scores in the three groups were (3.47 ± 1.20),(6.54 ±0.51),(0.21 ±0.07) point.Compared with control group,the differences of the factors in model group were statistically significant (all P ＜0.05).Compared with model group,the differences of the factors in experimental group were statistically significant (all P ＜ 0.05).Conclusion EGCG can ameliorate inflammation in pancreases and lungs through inhibition of NF-κB signaling pathways and improvement of oxygen free radical scavenging.