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Objective: To investigate the effect of arsenic sulfide (tetra-arsenic tetra-suffide As_4S_4; diarsenic trisulfideAs_2S_3)on tissue factor (TF) expression and procoagulant activity (PCA) of acute promyelocytic leukemia(APL) celllines (NB_4 and MR_2) and the basic mechanism of their role. Methods: NB_4 and MR_2 cells were respectively treatedwith As_4S_4, As_2S_3, As_4S_4 and Cyclohexamide(CHX). PCA of the cells was detected using one-stage clotting assay.TF antigen was detected by ELISA. TF and PML/RARα fusion gene mRNA by semi-quantitive RT-PCR. The PCA andTF antigen of HL-60 and K562 cells were also examined. Results: The PCA and TF antigen level in NB_4 and MR_2cells were significantly higher than that in HL-60 and K562 cells. Both As_4S_4 and As_2S_3 can down-regulate the TF anti-gen, TF mRNA transcription and membrane PCA of NB_4 and MR_2 cells in vitro in a time-dependent manner. The roleof As_4S_4 was stronger than that of As_2S_3. Both As_4S_4 and As_2S_3 had no effect on PML/RAR_α fusion gene
Objective: To investigate the effect of arsenic sulfide (tetra-arsenic tetra-suffide As_4S_4; diarsenic trisulfideAs_2S_3) on tissue factor (TF) expression and procoagulant activity (PCA) of acute promyocytic leukemia (APL) celllines (NB_4 and MR_2) and the basic The PCA of the cells was detected using one-stage clotting assay. TF antigen was detected by ELISA. TF and PML / RARα The PCA and TF antigen levels in NB_4 and MR_2 cells were significantly higher than those in HL-60 and K562 cells Both As_4S_4 and As_2S_3 can down-regulate the TF anti-gen, TF mRNA transcription and membrane PCA of NB_4 and MR_2 cells in vitro in a time-dependent manner. The roleof As_4S_4 was stronger than that of As_2S_3. Both As_4S_4 and As_2S_3 had no effect on PML / RAR_α fusion gene