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目的:研究探讨钴-原卟啉对大鼠脑损伤的保护作用。方法:用钴-原卟啉灌(50mg/kg)胃处理后,建立液压脑损伤模型,采用免疫组织化学SP三步法检测大鼠脑损伤区域血红素氧合酶-1(HO-1)的表达情况,用干湿重法测定损伤周边区脑含水量的变化。结果:正常组、假手术组大鼠HO-1均无表达;模型组脑损伤区域的HO-1阳性细胞数为(3.45±047);实验组12h、24h、3d和7dHO-1的阳性细胞数分别为:(10.9±1.35)、(10.62±1.88)、(12.4±1.57)和(10.99±2.42)。正常组、假手术组脑损伤周边区脑含水量分别为(78.7±0.4)%和(78.6±0.7)%;模型组脑含水量(89.3±0.3)%;实验组12h、24h、3d和7d脑含水量分别为:(83.1±0.3)%、(83.6±0.6)%、(83.9±0.4)%和(83.2±0.3)%。结论:钴-原卟啉(CoPP)能够诱导大鼠脑损伤区域HO-1的表达,减轻脑损伤周边区域的水肿程度,故钴-原卟啉对大鼠脑损伤具有保护作用。
Objective: To investigate the protective effect of cobalt-protoporphyrin on brain injury in rats. Methods: The model of hydraulic brain injury was established after the stomach was treated with cobalt-protoporphyrin (50 mg / kg), and the expression of HO-1 was detected by immunohistochemical SP three-step method. The changes of brain water content in the peripheral area of the lesion were measured by dry-wet method. Results: HO-1 was not expressed in the normal group and sham-operation group. The number of HO-1 positive cells in the model group was (3.45 ± 047). The positive cells of HO-1 in the experimental group at 12h, 24h, 3d and 7d The numbers were (10.9 ± 1.35), (10.62 ± 1.88), (12.4 ± 1.57) and (10.99 ± 2.42), respectively. The brain water content in the normal and sham-operated groups were (78.7 ± 0.4)% and (78.6 ± 0.7)%, respectively. The brain water content in the model group was 89.3 ± 0.3% The brain water contents were (83.1 ± 0.3)%, (83.6 ± 0.6)%, (83.9 ± 0.4)% and (83.2 ± 0.3)%, respectively. CONCLUSION: CoPP can induce the expression of HO-1 in brain injury area of rats and lessen the degree of edema in the peripheral area of brain injury. Therefore, cobalt-protoporphyrin has a protective effect on brain injury in rats.