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目的探讨CO2气腹对兔肝VX2肿瘤MMP2及血管新生的影响。方法采用组织块移植法建立兔肝 VX2肿瘤模型30只，随机分成3组，即气腹组(A组，n=10)、基质金属蛋白酶抑制剂(MMPi)组(B组，n=10)及免气腹组(C组，n=10)，模拟腹腔镜手术环境。免疫组织化学方法检测肿瘤及肿瘤旁组织MMP2及CD34的表达，采用IPP图像分析软件分析其平均光密度。结果 MMP2表达：A组、B组、C组肿瘤组织 MMP2平均光密度分别为0.2052±0.0167，0.1980±0.0332，0.1711±0.0284(P=0.022)；肿瘤旁组织 MMP2平均光密度分别为0.2081±0.0199，0.1726±0.0073，0.1396±0.0079(P=0.000)。CD34表达：A组、B组、C组肿瘤组织CD34平均光密度分别为0.2310±0.0226，0.2219±0.0154，0.1886±0.0180(P=0.000)；肿瘤旁组织CD34平均光密度分别为0.2198±0.0505，0.1761±0.0237，0.1108±0.0046(P=0.000)。结论 CO2气腹可通过 MMP2促进兔肝VX2肿瘤血管新生。“,”Objective To explore the effect of CO2 pneumoperitoneum on expression of MMP2 and angiogenesis of VX2 tumor in rabbit liver.Methods Thirty male New Zealand white rabbits with implanted liver VX2 tumors were randomly divided into three groups,Group A (Pneumo- peritoneum group, n=10), Group B (MMPi group, n=10) and Group C (Pneumo- peritoneum-free group, n=10). All groups received sham laparoscopic operation. Detected MMP2 and CD34 expressions both in tumor tissue and paratumor tissue by immunohisto- chemistry stain. Their mean optical density were analyzed by IPP system (Image-pro-plus 5.0.2). Results Expressions of MMP2 protein:In Group A, B, C, respectively, the mean optical density was 0.2052 ±0.0167,0.1980 ±0.0332,0.1711 ±0.0284 (P=0.022) in tumor tissue and 0.2081 ±0.0199, 0.1726 ±0.0073, 0.1396 ±0.0079 (P=0.000) in paratumor tissue. Expressions of CD34 protein:In Group A, B, C, respectively, the mean optical density was 0.2310±0.0226, 0.2219±0.0154, 0.1886± 0.0180 (P=0.000) in tumor tissue and 0.2198 ±0.0505, 0.1761 ±0.0237, 0.1108 ±0.0046 (P=0.000) in paratumor tissue. Conclusion CO2 pneumoperitoneum stimulates angiogenesis of VX2 tumor in rabbit liver through the change of expressions of MMP2.