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目的研究芒果核提取物动物体内外抑菌效果及其使用的安全性。方法采用二倍稀释法及琼脂扩散法,观察芒果核提取物对常见肠道感染致病菌(标准和耐药)志贺痢疾杆菌、福氏痢疾杆菌、大肠埃希菌的体外抑菌作用;采用小鼠复制感染模型观察芒果核提取物动物体内抑菌效果;用小鼠空腹经口灌胃给予芒果核提取物做急性毒性试验。结果芒果核提取物对标准和耐药志贺痢疾杆菌、福氏痢疾杆菌、大肠埃希菌有不同程度体外抑菌作用,其最低抑菌浓度(MIC)分别为7.8、7.8、7.8、15.6、15.6mg/ml和15.6mg/ml,抑菌圈直径分别为20、18、21、19、17、18mm;动物体内大、中、小剂量对(标准及耐药组)福氏痢疾杆菌及志贺痢疾杆菌感染小鼠有明显抑菌作用;小鼠灌胃最大耐受量40g/kg,相当临床用量的50倍。结论芒果核提取物对常见3种肠道感染菌具有较明显的体内外抑菌作用及具有良好的安全性。
Objective To study the in vitro and in vivo antibacterial effects and safety of mango nuclear extracts. Methods Two-fold dilution method and agar diffusion method were used to observe the antibacterial effects of mango nuclear extracts against common enteropathogenic pathogens (standard and resistant) Shigella dysenteriae, Shigella flexneri, and Escherichia coli. A mouse model of replicating infection was used to observe the antibacterial effect of the mango nucleus extract in vivo. The acute toxicity test of the mango nucleus extract was given by oral gavage. Results The mango nucleus extract had different degrees of in vitro antibacterial activity against the standard and resistant Shigella dysenteriae, Shigella flexneri, and Escherichia coli, and its minimum inhibitory concentration (MIC) was 7.8, 7.8, 7.8, and 15.6, respectively. At 15.6 mg/ml and 15.6 mg/ml, the diameters of the zones of inhibition were 20, 18, 21, 19, 17, and 18 mm, respectively; large, medium, and low doses of the animals (standard and resistant groups) were used for Shigella flexneri and The mice infected with Haemophilus dysenteriae had significant antibacterial effect; the maximum tolerated dose was 40 g/kg in mice, which was equivalent to 50 times the clinical dosage. Conclusion The mango nucleus extract has obvious bacteriostatic effect in vivo and in vitro and has good safety for three common intestinal tract infections.