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目的研究诱捕受体3(DcR3)蛋白在鼻咽癌中表达的临床意义,并探讨在鼻咽癌组织中DcR3与EB病毒(EBV)的关系。方法采用免疫组化SABC法检测40例鼻咽鳞状细胞癌组织中DcR3蛋白的表达情况,同时应用癌旁正常组织和40例鼻咽正常组织做对照。并用逆转录聚合酶链反应(RT-PCR)检测40例鼻咽癌组织中EBV的阳性率,探讨两者之间的关系。结果鼻咽癌组织中DcR3蛋白的阳性表达明显高于癌旁正常组织及正常鼻咽组织(P<0.05),癌旁正常组织与正常鼻咽组织中DcR3的表达差异无统计学意义(P>0.05);DcR3的表达与淋巴结转移、临床分期、病理分级有关(P<0.05),与患者年龄和性别无关(P>0.05);鼻咽癌组织中EBV的阳性率为72.50%,DcR3在EBV阳性的鼻咽癌组织中呈高表达,在鼻咽癌阴性的组织中呈低表达,差异有统计学意义(P<0.05)。结论 DcR3蛋白在鼻咽癌组织的高表达,可作为判断鼻咽癌分化、浸润、转移的重要指标,其与EBV共同作用促进了鼻咽癌的发生、转移及不良预后。
Objective To investigate the clinical significance of decoke receptor 3 (DcR3) protein expression in nasopharyngeal carcinoma and to explore the relationship between DcR3 and Epstein-Barr virus (EBV) in nasopharyngeal carcinoma. Methods Immunohistochemical SABC method was used to detect the expression of DcR3 protein in 40 cases of nasopharyngeal squamous cell carcinoma. Meanwhile, normal tissues adjacent to the tumor and 40 normal tissues of nasopharynx were used as controls. The positive rate of EBV in 40 cases of nasopharyngeal carcinoma was detected by reverse transcription-polymerase chain reaction (RT-PCR), and the relationship between the two was explored. Results The positive expression of DcR3 protein in nasopharyngeal carcinoma was significantly higher than that in adjacent normal tissues and normal nasopharyngeal tissues (P <0.05), and there was no significant difference in DcR3 expression between adjacent normal tissues and normal nasopharyngeal tissues (P> 0.05). The expression of DcR3 was correlated with lymph node metastasis, clinical stage and pathological grade (P <0.05), but not with age and sex (P> 0.05). The positive rate of EBV in nasopharyngeal carcinoma was 72.50% Positive nasopharyngeal carcinoma tissues were highly expressed in nasopharyngeal carcinoma negative tissue was low expression, the difference was statistically significant (P <0.05). Conclusion The high expression of DcR3 protein in nasopharyngeal carcinoma tissue can be used as an important index to judge the differentiation, invasion and metastasis of nasopharyngeal carcinoma. Combined with EBV, it promotes the occurrence and metastasis of nasopharyngeal carcinoma and its poor prognosis.