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目的探讨高盐高蛋白饮食对Dah1/SS大鼠血压、肾脏和消化道病理变化的影响。方法将30只SPF级雄性Dah1/SS大鼠随机分为3个组,即对照组、高盐组和高蛋白组,分别给予0.4%Na Cl普通低盐饲料、8%Na Cl高盐饲料和2.4%蛋氨酸饲料,喂养2个月,每周测量体质量及收缩压1次。实验结束,取大鼠肾脏、食管、胃、小肠、直肠及胰腺经HE染色后进行病理学观察。结果高盐、高蛋白组大鼠体质量增长明显低于对照组;高盐组大鼠收缩压高于对照组和高蛋白组大鼠,差异有统计学意义,高蛋白组大鼠未见血压升高;高盐及高蛋白组大鼠出现了不同程度的肾功能损伤,高盐组大鼠出现了肾脏病理性损伤;高盐和高蛋白饮食未见消化道及胰腺损伤。结论 Dah1/SS大鼠高盐饮食可成功建立盐敏感性高血压模型,且高盐高蛋白饮食对肾脏会造成不同程度的损伤,而对消化道及胰腺未造成损伤。
Objective To investigate the effects of high-salt, high-protein diet on pathological changes of blood pressure, kidneys and digestive tract in Dah1 / SS rats. Methods Thirty SPF male Dah1 / SS rats were randomly divided into three groups: control group, high salt group and high protein group, which were given 0.4% NaCl low salt diet, 8% NaCl high salt diet and 2.4% methionine feed, feeding for 2 months, weekly measurement of body mass and systolic blood pressure 1 times. At the end of the experiment, the kidneys, esophagus, stomach, small intestine, rectum and pancreas of rats were stained with HE for pathological observation. Results The body weight of rats in high salt and high protein group was significantly lower than that in control group. The systolic blood pressure in high salt group was higher than that in control group and high protein group Increased in high salt group and high protein group rats, while kidney damage occurred in high salt group and high salt group. No damage to digestive tract and pancreas was found in high salt and high protein diet. Conclusion Salt hypersensitivity model can be successfully established in high salt diet of Dah1 / SS rats, and high salt and high protein diet can cause different degrees of damage to the kidneys and no damage to digestive tract and pancreas.