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为了深入理解抗生素在土壤-植物系统中的迁移过程与规律,以土霉素(OTC)为目标抗生素,用水培蔬菜的方式,通过测定根际暴露24 h内3种常见蔬菜(上海青、木耳菜和快菜)叶中OTC含量的变化,计算OTC的叶累积速率;通过对比根际暴露24 h后OTC在植物体不同部位中的浓度分布,研究了OTC的迁移规律。结果表明,水溶液中的OTC能够被蔬菜根部快速吸收并传输到叶,根际暴露1 h内即可在叶中检出较高含量的OTC,且3种蔬菜叶中OTC的累积速率随暴露时间增加而降低。根际暴露24 h后,3种蔬菜叶中OTC的浓度大小依次为:木耳菜>快菜>上海青。分别在去离子水和植物根际液配置的含OTC营养液中进行24 h根暴露试验后,OTC在上海青不同部位中的浓度大小依次为:根>叶>茎和叶>根>茎,而在木耳菜不同部位中的浓度均为:叶>根>茎,在快菜叶中OTC浓度均大于根中浓度,植物根际分泌物可能影响其他植物对OTC的吸收与传输。
In order to understand the process and rules of antibiotic migration in soil-plant system, OTC was selected as the target antibiotic and cultured in hydroponics. Three kinds of common vegetables (Shanghai Green, Edible fungus Vegetable and fast food) leaf OTC content changes, calculate the OTC leaf accumulation rate; by comparing 24 hours after rhizospheric exposure OTC concentrations in different parts of the plant body distribution, study OTC migration rules. The results showed that the OTC in aqueous solution was quickly absorbed by the root of vegetables and transmitted to the leaves. Within 1 h after rhizosphere exposure, OTC was detected in the leaves, and the accumulation rate of OTC in three kinds of vegetable leaves varied with the exposure time Increase and decrease. After 24 h exposure to rhizosphere, the concentrations of OTC in three kinds of vegetable leaves were as follows: Acorus ear fungus> Fast food> Shanghai Qing. The results showed that the concentrations of OTC in different parts of P. euchroma were: root> leaf> stem and leaf> root> stem, and root exudates of OTC in 24 h after exposure to deionized water and plant rhizosphere solution respectively. However, the concentrations of OTC in leaves of different parts of A.leaves were leaf> root> stem, and the concentrations of OTC in fast leaf were higher than those in roots. The plant rhizosphere secretion may affect the absorption and transport of OTC by other plants.