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目的了解北京首例输入性ST-11 complex C群流行性脑脊髓膜炎(流脑)死亡病例流行病学特征及分离的ST-11 complex C群脑膜炎奈瑟菌株病原学特征。方法采集疑似病例的血液标本,进行脑膜炎奈瑟菌的分离培养、细菌学鉴定,确定为脑膜炎奈瑟菌;对分离菌株进行血清学分群、实时荧光定量PCR核酸鉴定,同时对鉴定的菌株进行多位点序列分型(MLST)分析、外膜蛋白PorA、fetA基因检测和体外药物敏感试验。结果经细菌学、血清学和实时荧光定量PCR鉴定,该菌株为脑膜炎奈瑟菌C血清群;porA为P1.5-1,10-8,fetA为F3-6;多位点序列分型分析表明,该菌株基因序列型(ST)为ST-2724,属于ST-11/ET-37克隆系;该菌株对氨苄西林和复方新诺明为中等耐药,对其他10种抗菌药物均敏感。结论该菌株为脑膜炎奈瑟菌C:P1.5-1,10-8:F3-6,序列型是ST-2724,属ST-11/ET-37克隆系,对常用抗菌药物尚未出现耐药现象。这是我国首次分离到的ST-11/ET-37克隆系C群脑膜炎奈瑟菌株,为输入性,提示应加强流脑病原学监测。
Objective To investigate the epidemiological characteristics of the first imported ST-11 complex group C meningitis (meningitis) and the etiological characteristics of the isolated ST-11 complex C group Neisseria meningitidis in Beijing. Methods The blood samples of suspected cases were collected for the isolation and culture of Neisseria meningitides and identified as Neisseria meningitidis. The isolates were identified by serological grouping and real-time fluorescence quantitative PCR, and the identified strains Multiple site sequence typing (MLST) analysis, outer membrane protein PorA, fetA gene test and in vitro drug sensitivity test. The results were confirmed by bacteriological, serological and real-time fluorescent quantitative PCR, which was serogroup C of Neisseria meningitidis; porA was P1.5-1,10-8, fetA was F3-6; multilocus sequence typing The results showed that ST-2724 was ST-2724 and belongs to ST-11 / ET-37 clones. The strain was moderately resistant to ampicillin and cotrimoxazole and sensitive to the other 10 antibacterials . Conclusion The strain is Neisseria meningitidis C: P1.5-1, 10-8: F3-6 and the sequence is ST-2724, belonging to ST-11 / ET-37 clone system. Drug phenomenon. This is the first isolate of Neisseria meningitides serogroup C in ST-11 / ET-37 clones in our country. It is an input that suggests that meningococcal etiology should be strengthened.