生物效价测定是抗生素原料、中间体及成品效价常用的测定方法 ,该法灵敏度高、专属性强、微量抗生素 (0 5Ｕ/ｍｌ)即可检出 ,与临床应用相符。生产单位及药检部门常采用二剂量法测定效价 ,本法因操作繁琐 ,细节多、试验过程长 ,培养时间需 16— 18小时 ,常有异常情况出现。以 The determination of biological potency is a commonly used method for the determination of the antibiotic raw materials, intermediates and finished products. The method has high sensitivity and specificity and can be detected with trace antibiotics (0 5U / ml), which is consistent with clinical application. Production units and drug testing departments often use two dosimeters to determine the titer, the law due to cumbersome operation, details and more, the test process is long, the training time to be 16-18 hours, often anomalies. To