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目的研究人转铁蛋白（HuTf）与金黄色葡萄球菌肠毒素B（SEB）偶联后，其Tf－SEB偶合蛋白的抗肿瘤活性，探讨Tf作为载体用于肿瘤导向免疫治疗的可能性。方法利用凋亡细胞指数检测和超抗原依赖的细胞介导的细胞毒（SDCC）效应，观察该偶合蛋白的起抗原活性和激活T细胞杀伤肿瘤细胞的作用。结果Tf－SEB偶合蛋白比单体SEB能更有效地激活T细胞的杀伤肿瘤细胞活性，并对MHC－Ⅱ类分子阴性的肿瘤靶细胞也具有较强的杀伤效果。结论Tf作为载体应用于肿瘤的导向治疗具有可行性。
Objective To investigate the anti-tumor activity of Tf-SEB-coupled protein after human transferrin (HuTf) was conjugated to S. aureus enterotoxin B (SEB) and explore the possibility of using Tf as a vector for tumor-directed immunotherapy. Methods Apoptotic cell index assay and superantigen-dependent cell-mediated cytotoxicity (SDCC) effect were used to observe the antigenic activity of the coupled protein and activate the T cells to kill tumor cells. Results The Tf-SEB-coupled protein was more effective than the monomeric SEB in activating T-cell killing activity of tumor cells, and it also had a strong killing effect on tumor target cells that were negative for MHC-II molecules. Conclusion Tf can be used as a carrier for tumor-oriented therapy.