论文部分内容阅读
目的研究胆囊腺癌、癌旁组织和慢性胆囊炎组织中NF-κB/p65表达水平及其临床病理意义。方法108例胆囊腺癌、46例癌旁组织和35例慢性胆囊炎手术切除标本常规石蜡包埋切片,NF-κB/p65染色方法为EnVisionTM免疫组化法。结果胆囊腺癌NF-κB/p65表达阳性率及其评分明显高于癌旁组织(χ2=10.58,t=3.39,P<0.01)和慢性胆囊炎(χ2=14.98,t=3.76,P<0.01);腺瘤癌变、肿块最大径<2 cm、无淋巴结转移和未侵犯周围组织病例NF-κB/p65表达阳性率及其评分明显低于低分化黏液腺癌、肿块最大径≥2 cm、淋巴结转移及侵犯周围组织病例(P<0.05或P<0.01)。结论NF-κB/p65表达可能是反映胆囊腺癌发生、进展、生物学行为和预后的重要生物学标记物。
Objective To study the expression of NF-κB / p65 in gallbladder adenocarcinoma, paracarcinoma and chronic cholecystitis and its clinicopathological significance. Methods 108 cases of gallbladder adenocarcinoma, 46 cases of paracancerous tissues and 35 cases of chronic cholecystitis were paraffin-embedded sections. NF-κB / p65 staining was EnVisionTM immunohistochemistry. Results The positive rates and scores of NF-κB / p65 in gallbladder adenocarcinoma were significantly higher than those in adjacent non-cancerous tissues (χ2 = 10.58, t = 3.39, P <0.01) and chronic cholecystitis (χ2 = 14.98, ). The positive rate and score of NF-κB / p65 in lymph node metastasis and non-infiltrating surrounding tissues were significantly lower than that in poorly differentiated mucinous adenocarcinoma, the maximum diameter of tumor was less than 2 cm, lymph node Metastasis and invasion of surrounding tissue cases (P <0.05 or P <0.01). Conclusion The expression of NF-κB / p65 may be an important biological marker reflecting the occurrence, progression, biological behavior and prognosis of gallbladder adenocarcinoma.