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目的以百草枯和白藜芦醇为受试物,以大鼠RLE-6TN细胞为研究对象,研究百草枯对肺泡Ⅱ型上皮细胞所造成的氧化损伤,同时探讨白藜芦醇的抗氧化作用能否拮抗百草枯对肺泡Ⅱ型上皮细胞所造成的氧化损伤而起到保护作用。方法检测1 000μmol/L百草枯组和2个白藜芦醇保护组(60和80μmol/L)的细胞活性、超氧化物歧化酶(SOD)活性、乳酸脱氢酶(LDH)活性、细胞凋亡情况和DNA的损伤程度。结果百草枯组、60μmol/L白藜芦醇保护组和80μmol/L白藜芦醇保护组的细胞活性分别为50.88%、63.29%和69.27%;SOD活性分别为(111.8±2.11)U/ml、(122.4±1.20)U/ml和(127.4±2.42)U/ml;LDH活性分别为(25.2±2.29)U/L、(16.8±1.32)U/L和(13.8±2.29)U/L;细胞凋亡率分别为6.51%、5.70%和5.15%;DNA受损的细胞率分别为35%、22%和19%。结论 1 000μmol/L百草枯可对RLE-6TN细胞造成严重的氧化损伤,引起超氧化物歧化酶活力下降和乳酸脱氢酶活力的升高,诱导细胞凋亡发生,造成细胞DNA损伤。白藜芦醇能改善百草枯对RLE-6TN细胞造成的氧化损伤,降低细胞的凋亡率,减轻细胞DNA损伤。
OBJECTIVE To study the oxidative damage caused by paraquat to alveolar type Ⅱ epithelial cells by using paraquat and resveratrol as test substance and rat RLE-6TN as research object, and to explore the antioxidative effect of resveratrol Can antagonize paraquat on alveolar type Ⅱ epithelial cells caused by oxidative damage and play a protective role. Methods The cell viability, superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity in 1 000 μmol / L paraquat and 2 resveratrol groups (60 and 80 μmol / L) Death and DNA damage. Results The cell viability of paraquat group, 60μmol / L resveratrol and 80μmol / L resveratrol groups were 50.88%, 63.29% and 69.27%, respectively. The SOD activity was (111.8 ± 2.11) U / ml , (122.4 ± 1.20) U / ml and (127.4 ± 2.42) U / ml respectively; LDH activities were (25.2 ± 2.29) U / L, (16.8 ± 1.32) U / L and (13.8 ± 2.29) U / The apoptotic rates were 6.51%, 5.70% and 5.15%, respectively. The rates of DNA damage were 35%, 22% and 19% respectively. Conclusion Paraquat at 1 000 μmol / L can cause severe oxidative damage to RLE-6TN cells, which leads to the decrease of superoxide dismutase activity and the increase of lactate dehydrogenase activity, induces cell apoptosis and causes DNA damage. Resveratrol can improve the oxidative damage caused by paraquat to RLE-6TN cells, reduce the apoptosis rate of cells and reduce the DNA damage of cells.