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以小白菜带柄子叶为外植体,通过四种细胞分裂素BA,KT,ZT,2iP的不同浓度及配比,诱导带柄子叶直接产生不定芽。结果表明,单独使用较高浓度的各种细胞分裂素都能诱导不定芽的形成。其中采用两种细胞分裂素降低浓度如BA2mg·L-1和KT1mg·L-1配合使用可以提高不定芽的频率达41.58%。另外增加适当浓度的AgNO34mg·L-1和ABA0.2mg·L-1能促进不定芽的发生达50%。试验发现在含有萘乙酸(NAA),吲哚乙酸(IAA),吲哚丁酸(IBA),生根粉(ABT),土菌消(HMI)这五种生根培养基中,吲哚了酸和土菌消对小白菜幼苗生根最为有利。本试验表明,小白菜离体培养再生植株是通过直接出芽的方式比通过愈伤组织再生芽的方式缩短了生长周期,而且明显提高了再生芽的频率。
Taking the cotyledons of Chinese cabbage as explants, adventitious buds were induced directly by the cotyledons with different concentration and ratio of four cytokinins, BA, KT, ZT and 2iP. The results showed that adventitious bud formation could be induced by using higher concentrations of various cytokines alone. The use of two cytokinin reducing concentrations such as BA2mg · L-1 and KT1mg · L-1 could increase the frequency of adventitious buds by 41.58%. Addition of appropriate concentrations of AgNO34mg · L-1 and ABA0.2mg · L-1 could promote adventitious shoots up to 50%. It was found that in the five rooting medium containing NAA, IAA, IBA, ABT and HMI, Soil bacteria eliminate cabbage seedling rooting the most favorable. The results showed that the in vitro culture of Brassica chinensis reduced the growth cycle by direct budding and regenerated buds through callus, and significantly increased the frequency of regenerated buds.