论文部分内容阅读
将磁性纳米微球(MNP,表面修饰羧基的磁性四氧化三铁微球)与表面等离子体共振(SPR)免疫传感技术结合,以莠去津单克隆抗体(AT-m Ab)与磁性纳米微球的偶联物(AT-m AbM NP)作为传感识别元件,初步建立了一种用于饮用水中除草剂莠去津残留检测的SPR信号增强免疫传感方法。通过对检测条件的优化,该方法对自来水中莠去津的检出限为0.89 ng/m L(S/N=3),检测范围为8.62~7.18×10~3ng/m L,检测时间小于20 min;在10~1 000 ng/m L添加水平内,莠去津的平均回收率为94%~102%,相对标准偏差(RSD)为5.1%~7.3%。磁性纳米微球的加入有效增强了SPR传感器的响应信号强度,提高了检测方法的灵敏度。本研究建立了一种快速、灵敏、准确的水中莠去津残留的检测方法,可为相应的现场检测技术和设备的研发提供技术基础。
Magnetic nanoparticle (MNP, surface modified carboxyl group magnetic ferriferrite microspheres) was combined with surface plasmon resonance (SPR) immunosensor technology. Atrazine monoclonal antibody (AT-m Ab) A conjugate of microspheres (AT-m AbM NP) was used as a sensing identification element to establish an SPR signal enhanced immunosensing method for the determination of atrazine residue in drinking water. The detection limit of atrazine in tap water was 0.89 ng / m L (S / N = 3). The detection range was 8.62 ~ 7.18 × 10 ~ 3 ng / m L, and the detection time was less than 20 min. The average recoveries of atrazine ranged from 94% to 102% and the relative standard deviations (RSDs) ranged from 5.1% to 7.3% at 10-1 000 ng / mL. The addition of magnetic nanospheres effectively enhances the response signal intensity of the SPR sensor and enhances the sensitivity of the detection method. This study established a rapid, sensitive and accurate detection of atrazine residues in water, which can provide technical basis for the corresponding field detection technology and equipment development.