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目的:观察大鼠单眼开放性眼外伤对双眼的影响,为伤眼及对侧眼的病理组织学观察提供双侧对比的直接依据。方法:44只大鼠随机分为4组,眼外伤组各12只,正常对照组8只。单纯穿通伤组以右眼颞上10点位为中心距角巩膜缘后1.0mm做与其平行的弧形切口,长约2mm,深达玻璃体腔,切口处造成色素膜嵌顿,色素膜外露部分与切口缘平行,切口不予缝合;异物伤组在此基础上于切口处分别置入玻璃体腔1.0mm×1.5mm大小长方形双面刀片或1.0mm×1.0mm×1.0mm大小石粒一枚。致伤后不予处置,观察眼部表现,四组内再随机均分为四小组,分别于致伤后2、4、6、8周处死。放大镜下摘除双眼眼球,冰冻切片,HE染色,数码显微镜下观察病理改变。结果:单纯穿通伤组伤眼在2周时表现为视网膜、脉络膜急性炎症;4周始观察到脉络膜增生肥厚、单核细胞浸润的慢性炎症特点;8周时可见1例眼球萎缩。异物伤组伤眼病理组织学在2周时均表现为急性化脓性炎症;4周时观察到脉络膜慢性炎症改变的程度较单纯穿通伤组重;6周始视网膜、脉络膜的萎缩性改变明显;8周时大部分伤眼眼球萎缩。结论:(1)眼内异物存留是发生化脓性眼内炎的危险因素。(2)单纯穿通伤及球内异物伤合并色素膜嵌顿8周内未见伤眼及对侧眼任何交感性眼炎的典型及非典型病理改变。
OBJECTIVE: To observe the effect of unilateral ocular traumatic eye injury on both eyes in order to provide a direct basis for bilateral comparison for the pathological observation of injured and contralateral eyes. Methods: Forty-four rats were randomly divided into 4 groups: 12 eyes in each group and 8 eyes in normal control group. Simple penetrating injury group to the right eye temporal superior 10 o’clock position as the center of the scleral margin 1.0mm to do parallel with its curved incision, about 2mm, deep into the vitreous cavity, the incision at the pigmented incarcerated, pigmented film exposed part And incision edge parallel to the incision not be sutured; foreign body injury group on the basis of these were placed in the vitreous cavity 1.0mm × 1.5mm size rectangular double-sided blade or 1.0mm × 1.0mm × 1.0mm size of a stone. Disposal after injury, observe the performance of the eye, the four groups were randomly divided into four groups randomly, were killed 2,4,6,8 weeks after injury. Eyes under the magnifying glass removal, frozen sections, HE staining, observed under a digital microscope pathological changes. Results: The traumatic eyes of pure penetrating injury group showed retinal and choroidal acute inflammation at 2 weeks. Choroidal hyperplasia and chronic inflammatory infiltration of mononuclear cells were observed at 4 weeks. One case showed atrophy of the eyeball at 8 weeks. At the 2nd week, the histopathology of the injured eye showed acute suppurative inflammation. At 4 weeks, chronic choroidal inflammation was observed more severe than that of the pure penetrating injury group. At 6th week, the atrophic changes of the retina and choroid changed obviously. After 8 weeks Most traumatic eye atrophy. Conclusion: (1) Intraocular foreign body retention is the risk factor for purulent endophthalmitis. (2) The typical and atypical pathological changes of penetrating injury and intraocular foreign body injury with pigmented incontinence in 8 weeks without any injury to the eyes and any sympathetic ophthalmia in the contralateral eye.