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微卫星是近年大力开发的一种分子标记,为了推进赤拟谷盗Tribolium castaneum(Herbst)遗传学相关研究,对赤拟谷盗全基因组和EST中由1~6个碱基重复单元组成的简单序列重复进行分析,进而对其微卫星的丰度和分布进行比较分析。微卫星在赤拟谷盗EST中的分布频率为1/0.87kb,其中单碱基重复序列占71.25%,是最丰富的重复单元,而六、三、四、二,五碱基重复单元序列分别占23.93%,2.94%,1.56%,0.17%,0.15%。全基因组中微卫星的分布频率为1/3.65kb,其中六碱基重复序列占61.96%,是最丰富的重复单元,而三,四,一,五,二碱基重复单元序列分别占14.35%,13.75%,4.68%,3.60%,1.69%。同时发现富含A和T碱基的微卫星占主导地位,富含G和C碱基的微卫星数量较少。进一步的分析显示,微卫星在每条染色体上的丰度存在很大的相似性。
Microsatellite is a kind of molecular marker that has been vigorously developed in recent years. In order to promote the genetic study of Tribolium castaneum (Herbst), the microsatellite consists of 1 to 6 base repeat units Sequence repeated analysis, and then its microsatellite abundance and distribution of comparative analysis. The distribution frequency of microsatellites in ESTs was 1 / 0.87kb, of which 71.25% was single-base repeats and the most abundant repeat units. The six, three, four, two, five base repeat units Accounting for 23.93%, 2.94%, 1.56%, 0.17% and 0.15% respectively. The distribution frequency of the microsatellite in the whole genome is 1 / 3.65kb, in which the six-base repeat sequence occupies 61.96%, which is the most abundant repeat unit, while the third, fourth, first, fifth and second base repeat unit sequences account for 14.35% , 13.75%, 4.68%, 3.60%, 1.69%. At the same time, we found that the microsatellite containing A and T bases is dominant, and the number of microsatellites rich in G and C bases is less. Further analysis showed that the abundance of microsatellites in each chromosome there is a great similarity.