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目的:探索人多发性骨髓瘤裸鼠皮下移植瘤模型建立的方法。方法:Balb/c裸鼠进行3GyX线照射预处理后24h,于其皮下接种2×107人多发性骨髓瘤细胞RPMI8226,每2~3d观察裸鼠体重的变化、肿瘤生长的情况、裸鼠的生存时间等,待裸鼠濒死或死亡,或者超过观察时间仍未死亡时,处死裸鼠,取其皮下结节及器官进行病理检测,同时摘眼球取血行血清免疫固定电泳检测。结果:荷瘤裸鼠出现皮下结节的高峰为荷瘤后第2~3周,结节体积基本达到高峰为荷瘤后第5~6周;裸鼠死亡高峰出现在荷瘤后第7周,荷瘤小鼠中位生存时间为荷瘤后52d;裸鼠处死后血清中未检测到人源单克隆免疫球蛋白;皮下结节HE染色证实瘤组织为浆细胞来源,器官HE染色显示肝脏部分炎性损伤。结论:采用RPMI8226人骨髓瘤细胞于Balb/c裸鼠皮下接种(接种细胞数为2×107个细胞)造模的方法,具有操作过程较简单、技术要求低,观察肿瘤生长较为直观等优点;同时采用X线照射预处理的方法可降低裸鼠免疫力,明显提高模型的成瘤率;人多发性骨髓瘤细胞RPMI8226皮下接种于裸鼠,肿瘤生长过程中很难有人源单克隆免疫球蛋白分泌入血;荷瘤裸鼠出现肝脏的损伤,也许可作为今后抗肿瘤药物疗效研究的指标之一。
Objective: To explore a method for establishing a subcutaneous xenograft model of human multiple myeloma in nude mice. Methods: Balb / c nude mice were irradiated with 3GyX radiation for 24 hours and then subcutaneously inoculated 2 × 107 human multiple myeloma RPMI8226 cells. The body weight of nude mice was observed every 2 ~ 3d, the growth of the nude mice Survival time, etc., until the nude mouse dying or death, or more than the observation time has not died, the nude mice were sacrificed, and its subcutaneous nodules and organs for pathological examination, while taking the eyeball blood serum immunostaining electrophoresis. Results: The peak of subcutaneous nodules appeared in tumor-bearing nude mice at 2 to 3 weeks after tumor-bearing, and the nodule volume reached the peak at 5 to 6 weeks after tumor-bearing. The peak of death in nude mice occurred at the 7th week after tumor-bearing , And the median survival time of tumor-bearing mice was 52 days after tumor-bearing. No human monoclonal immunoglobulin was detected in the serum of nude mice after sacrifice; the subcutaneous nodules were stained with hematoxylin and eosin Partial inflammatory damage. CONCLUSION: The method of subcutaneously inoculating Balb / c nude mice with 2 × 107 cells inoculated with RPMI8226 human myeloma cells has the advantages of simple operation, low technical requirement, and more intuitive observation of tumor growth. At the same time, pretreatment with X-ray irradiation can reduce the immunity of nude mice and significantly increase the rate of tumorigenesis. The multiple myeloma RPMI8226 cells were inoculated subcutaneously in nude mice. It is difficult to have human monoclonal immunoglobulin Secretion into the blood; tumor-bearing nude mice liver damage, may be used as one of the indicators of the efficacy of anticancer drugs in the future.