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采用16S rDNA的PCR-DGGE基因指纹技术,于6~9月间,对辽宁普兰店湾2个相邻的刺参养殖池塘进行了养殖水环境中菌群多样性的分析,首先用2216E固体培养基对仿刺参养殖水环境中的细菌总数进行统计,再采用PCR-DGGE指纹技术分析菌群多样性。结果表明,池塘水环境中较高水平的细菌数,且9月为6月的3倍;高温季节刺参养殖水环境中细菌种群较为丰富,其中大多数属于变形菌门;6~9月份样品具有一致的优势菌群,分别为Pseudomonas sp.,Uncul-tureddelta bacterium,Ostreococcus sp.以及Unculturedalpha proteobacterium。同时发现,同一时间采集的样品,有一定的菌群相似性,并在聚类分析图中分别成簇,检测样品的细菌多样性指数在1.7至2.5之间。
Using 16S rDNA PCR-DGGE fingerprinting, from June to September, two adjacent sea cucumber breeding ponds in Pulandian Bay of Liaoning Province were used to analyze the flora diversity in aquaculture environment. Firstly, 2216E solid culture Based on the statistics of the total number of bacteria in the aquaculture environment of sea cucumber, the microbial community diversity was analyzed by PCR-DGGE fingerprinting. The results showed that the pond water environment in a higher number of bacteria, and in September 3 times in June; high temperature seabuckthorn breeding aquaculture environment is rich in bacteria, most of which belong to the proteobacteria; June to September samples Consistent with the dominant flora, respectively, Pseudomonas sp., Uncul- tureddelta bacterium, Ostreococcus sp., And Unculturedalpha proteobacterium. At the same time, it was found that the samples collected at the same time had some similarities of bacteria groups, and clustered respectively in the cluster analysis charts. The bacterial diversity index of the samples tested ranged from 1.7 to 2.5.