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目的优化五味子木脂素超声辅助提取工艺,获取工艺参数。方法利用响应面法(RSM)对五味子木脂素超声辅助乙醇提取工艺进行优化。在单因素实验基础上,选择乙醇浓度、超声提取时间和液固比为自变量,木脂素提取率为响应值,根据中心组合设计原理采用3因素5水平的响应面分析法,研究乙醇浓度、超声时间、液固比及其交互作用对五味子3种主要木脂素提取率的影响。应用SAS软件和响应面分析相结合的方法,模拟得到回归方程的预测模型和可信度,并通过岭脊分析得到最佳的提取条件。结果五味子木脂素超声辅助乙醇提取最佳条件为:乙醇浓度85%、超声时间34 min、液固比146 mL/g。该条件下3种主要木脂素类成分总提取率为1.13%。结论研究建立的模型适合五味子木脂素的提取,可用于实际生产。
Objective To optimize the ultrasonic-assisted extraction process of Schisandra lignans and obtain the process parameters. Methods Response surface methodology (RSM) was used to optimize the ultrasonic-assisted extraction of ethanol from Schisandra chinensis seeds. On the basis of single factor experiments, ethanol concentration, ultrasonic extraction time and liquid-solid ratio as independent variables and lignan extraction rate were selected as response values. According to the central composite design principle, response surface methodology with 3 factors and 5 levels was used to study the effect of ethanol concentration , Ultrasonic time, liquid-solid ratio and their interaction on the extraction rate of three main lignans from Schisandra chinensis. By using SAS software and response surface analysis, the prediction model and reliability of regression equation are simulated and the optimal extraction conditions are obtained by ridge ridge analysis. Results The optimum conditions of ultrasonic-assisted ethanol extraction of Schisandra lignans were: ethanol concentration 85%, ultrasonic time 34 min, liquid-solid ratio 146 mL / g. Under this condition, the total extraction rate of the three main lignan components was 1.13%. Conclusion The model established by this study is suitable for the extraction of Schisandra lignans and can be used in practical production.