论文部分内容阅读
目的研究肉桂醛对体外培养的人黑素瘤细胞株A375凋亡和血管内皮细胞生长因子(VEGF)及基质金属蛋白酶-9(MMP-9)表达的影响。方法 A375细胞在体外二维和三维培养后,在培养基中加入不同浓度(0,10,20和40μmol/L)肉桂醛,24小时后流式细胞仪检测细胞周期和凋亡,Western blot法检测VEGF蛋白水平,明胶酶谱法检测MMP-9分泌情况。结果肉桂醛能促进A375细胞凋亡,降低细胞增殖指数。40μmol/L肉桂醛作用后的A375细胞增殖指数最低(53.38±4.25%vs35.13±3.24%),细胞凋亡百分比最高(4.37±0.25%vs10.50±0.37%),各浓度间作用差异具有统计学意义(P<0.05)。肉桂醛作用后二维和三维培养的人黑素瘤细胞VEGF的表达下降(1.193±0.195vs0.204±0.017;1.549±0.214vs0.392±0.019),MMP-9的分泌也减少(24885.83±865.80vs14150.77±555.63;29408.50±817.62vs15121.43±497.95),各浓度肉桂醛间的抑制强度差异具有统计学意义(P<0.05)。结论肉桂醛能促进人黑素瘤细胞凋亡,抑制其增殖,并通过降低VEGF和MMP-9的表达来抑制人黑素瘤细胞的侵袭,其抑制强度与药物浓度成正比。
Objective To investigate the effect of cinnamaldehyde on the apoptosis of human melanoma cell line A375 and the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) in vitro. Methods A375 cells were cultured in vitro with different concentrations (0, 10, 20 and 40 μmol / L) of cinnamaldehyde after two and three dimensional in vitro culture. Cell cycle and apoptosis were detected by flow cytometry after 24 hours. Western blot Detect the level of VEGF protein, and detect the secretion of MMP-9 by gelatin zymography. Results Cinnamaldehyde can promote the apoptosis of A375 cells and decrease the cell proliferation index. A340 cells treated with 40μmol / L cinnamaldehyde had the lowest proliferation index (53.38 ± 4.25% vs35.13 ± 3.24%), the highest percentage of apoptotic cells (4.37 ± 0.25% vs10.50 ± 0.37%), Statistical significance (P <0.05). The expression of VEGF in two-dimensional and three-dimensional cultured human melanoma cells decreased after cinnamaldehyde treatment (1.193 ± 0.195vs 0.204 ± 0.017; 1.549 ± 0.214vs 0.39 ± 0.019), and the secretion of MMP-9 also decreased (24885.83 ± 865.80 vs14150.77 ± 555.63; 29408.50 ± 817.62vs15121.43 ± 497.95). There was a significant difference in the inhibitory strength between the cinnamic aldehyde groups (P <0.05). Conclusion Cinnamaldehyde can promote the apoptosis of human melanoma cells and inhibit the proliferation of human melanoma cells, and inhibit the invasion of human melanoma cells by decreasing the expression of VEGF and MMP-9. The inhibitory intensity is proportional to the drug concentration.