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甲状腺细胞原代培养是一种较新的实验手段,在甲状腺功能和甲状腺疾病的研究工作中具有重要意义。从原代取材到单细胞悬液制备的过程是甲状腺上皮细胞体外培养成功的第一步;采用不同培养介质可以使甲状腺细胞的生存时间延长、生理功能更强;甲状腺细胞的成功冷冻保存和复苏对体外研究甲状腺生理功能和病理变化也是有重要意义的。本文对国内外甲状腺上皮细胞的体外培养予以综述。
Primary culture of thyroid cells is a relatively new experimental method, which is of great significance in the research of thyroid function and thyroid diseases. The process of preparation from primary culture to single cell suspension is the first step in the success of thyroid epithelial cells culture in vitro. The use of different culture medium can prolong the survival time of thyroid cells and have more physiological functions. The successful cryopreservation and resuscitation of thyroid cells It is also important to study thyroid physiological function and pathological changes in vitro. In this paper, thyroid epithelial cells at home and abroad in vitro culture are reviewed.