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目的探讨甲胎蛋白单抗修饰的荷载人重组核心蛋白聚糖质粒的聚乳酸-羟基乙酸[alpha-fetoprotein monoclonal antibody-poly(lactic-co-glycolic acid)-recombinant human decorin,AFPm Ab-PLGA-rhDCN]纳米粒对肝癌细胞株HepG2细胞迁移和侵袭能力的影响。方法将HepG2细胞分为未处理组、用含空质粒的PLGA纳米粒处理的空白组及低、中、高浓度AFPmAb-PLGA-rhDCN纳米粒处理的实验组,采用划痕试验和Transwell小室试验检测纳米粒对细胞迁移和侵袭的影响,RT-PCR法检测RhoC基因mRNA转录水平,ELISA和Western blot法检测RhoC蛋白的表达水平。结果低、中、高浓度实验组细胞划痕宽度值及穿透膜的细胞数与未处理组及空白组相比,差异均有统计学意义(P均<0.05);低、中、高浓度实验组细胞RhoC基因mRNA灰度值及蛋白浓度均低于未处理组及空白组,差异均有统计学意义(P均<0.05)。结论 AFPm Ab-PLGA-rhDCN纳米粒对HepG2细胞的迁移和侵袭能力具有抑制作用,该抑制作用与下调RhoC分子的表达有关。
Objective To investigate the effect of alphafetoprotein monoclonal antibody-poly (lactic-co-glycolic acid) -recombinant human decorin, AFPm Ab-PLGA-rhDCN Effect of Nanoparticles on Migration and Invasion Ability of HepG2 Cells. Methods HepG2 cells were divided into untreated group, blank group treated with PLGA nanoparticles containing empty plasmid, and experimental group treated with low, medium and high concentrations of AFPmAb-PLGA-rhDCN nanoparticles. Scratch test and Transwell chamber assay The effects of nanoparticles on cell migration and invasion were analyzed by RT-PCR and RhoC protein expression levels by ELISA and Western blot. Results Compared with untreated group and blank group, the cell scratch width and the number of cells penetrating the membrane in the low, medium and high concentration groups were significantly different (all P <0.05). Low, medium and high concentration The gray value and protein concentration of RhoC gene in experimental group were lower than those in untreated group and blank group (all P <0.05). Conclusion AFPm Ab-PLGA-rhDCN nanoparticles can inhibit the migration and invasion ability of HepG2 cells, which is related to the down-regulation of RhoC expression.