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为探讨矮壮素(CCC)调控银杏叶萜内酯生物合成的机理,以3年生银杏实生苗为试材,研究了0、0.5、1.0和2.0 g.L-1CCC处理对银杏叶光合作用、光合色素、可溶性糖和萜内酯含量的影响,并采用实时定量PCR技术(qRT-PCR)检测了对银杏萜内酯合成途径中5个关键基因表达水平的影响。结果表明,0.5、1.0和2.0 g.L-1 CCC处理均可显著提高银杏叶光合作用速率,气孔导度,细胞间CO2浓度,蒸腾速率,以及叶绿素、类胡萝卜素和可溶性糖的含量。在1.0和2.0 g.L-1CCC处理下,银杏内酯A、银杏内酯B、白果内酯和总萜内酯含量显著高于对照。qRT-PCR分析结果显示CCC处理能显著上调银杏内酯合成途径中4个关键基因(DXS、DXR、GGPPS、LPS)的表达,表明CCC在分子水平上可能是通过诱导内酯合成关键基因表达来促进内酯的生物合成。
To investigate the mechanism of CCC regulating the biosynthesis of ginkgolides, the effects of 0, 0.5, 1.0 and 2.0 gL-1 CCC treatment on the photosynthesis, photosynthetic pigments , Soluble sugar and terpene lactone content. The effects of 5 key genes on the synthesis of ginkgolides were tested by real-time quantitative PCR (qRT-PCR). The results showed that 0.5, 1.0 and 2.0 g.L-1 CCC treatment significantly increased the photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, and the content of chlorophyll, carotenoid and soluble sugar. Under 1.0 and 2.0 g.L-1CCC treatment, the contents of ginkgolide A, ginkgolide B, bilobalide and total terpene lactones were significantly higher than those of the control. qRT-PCR analysis showed that CCC treatment up-regulated the expression of four key genes (DXS, DXR, GGPPS and LPS) in Ginkgolide synthesis pathway, indicating that CCC may be induced by lactone synthesis at key molecular level Promote lactone biosynthesis.