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Micro RNAs(miRNAs)在植物的生长发育以及植物对环境的适应方面发挥了重要的作用,成为近几年研究的热点。mi R172家族是一个保守的mi RNA家族,之前的研究表明mi R172的作用涉及生长期过渡、闭花受精、花器官发育、开花时间调控等方面。但是到目前为止,人们对大豆mi R172家族的了解还不是很清楚。本实验通过mi RBase、PLACE数据库及DNAman软件对gma-mi R172家族成员的前体序列、成熟序列、启动子序列进行比较分析,发现gma-mi R172家族成员成熟序列虽然高度保守,但前体序列具有一定的差异,这可能导致了各成员的功能差异;启动子分析显示其启动子含有光反应原件和多种非生物胁迫响应元件,推测其可能参与大豆的开花调控和逆境胁迫响应。通过实时定量PCR分析了大豆mi R172成员的组织特异性表达情况。利用PMRD数据库对gma-mi R172的靶基因进行了生物信息学分析,获得了7个AP2-like类转录因子,参与花器官发育、开花时间调控及非生物胁迫响应的方面。上述结果初步分析了大豆mi R172家族的功能与靶基因,对今后进一步分析研究大豆mi R172家族及其靶基因打下了基础。
MicroRNAs (miRNAs) play an important role in the growth and development of plants and the adaptation of plants to the environment, and become the hot spots in recent years. The mi R172 family is a conserved family of miRNAs. Previous studies have shown that mi R172 is involved in the transitional stages of growth, flowering insemination, floral organ development and flowering timing. But so far, people’s understanding of the soybean mi R172 family is still unclear. In this study, we compared the precursor, mature and promoter sequences of gma-mi R172 family member by mi RBase, PLACE database and DNAman software. It was found that although the mature sequence of gma-mi R172 family member is highly conserved, the precursor sequence Which may lead to functional differences among the members. Promoter analysis showed that its promoter contains photoreactions and a variety of abiotic stress response elements, presumably involved in the flowering and stress response of soybean. Tissue-specific expression of soybean mi R172 members was analyzed by real-time quantitative PCR. Bioinformatics analysis of the target gene of gma-mi R172 using PMRD database provided seven AP2-like transcription factors involved in floral organ development, flowering timing and abiotic stress response. The above results preliminarily analyzed the function and target genes of soybean mi R172 family, which laid the foundation for further analysis and study of the mi R172 family of soybean and its target genes in the future.