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目的:了解锌对人前列腺癌细胞增殖的影响。方法:采用不同浓度锌溶液干预人前列腺癌细胞株体外培养,测定细胞生长曲线,检测细胞生长抑制情况、细胞存活率、细胞凋亡及细胞周期情况。结果:锌对雄激素非依赖型(DU-145和PC-3)及雄激素依赖型(LNCaP)前列腺癌细胞均有抑制增殖和促进凋亡作用,对激素依赖型抑制更明显,3种细胞的IC50分别为780ng/ml、810ng/ml、100ng/ml。1000ng/ml锌对3种细胞生长均达最大抑制作用。随着锌浓度增高,3种细胞凋亡率均上升。结论:锌可以明显抑制雄激素非依赖型以及雄激素依赖型前列腺癌细胞的增殖,可能与锌诱导细胞凋亡有关。
Objective: To understand the effect of zinc on the proliferation of human prostate cancer cells. Methods: The human prostate cancer cell lines were treated with different concentrations of zinc solution in vitro, the cell growth curve was measured, the cell growth inhibition, cell viability, apoptosis and cell cycle were detected. RESULTS: Zinc inhibited the proliferation and promoted the apoptosis of androgen-independent (DU-145 and PC-3) and androgen-dependent (LNCaP) The IC50 were 780 ng / ml, 810 ng / ml, 100 ng / ml, respectively. The maximum inhibitory effect of 1000ng / ml zinc on the three kinds of cell growth was reached. With the increase of zinc concentration, the apoptosis rate of all three kinds of cells increased. Conclusion: Zinc can significantly inhibit the proliferation of androgen-independent and androgen-dependent prostate cancer cells, which may be related to zinc-induced apoptosis.