应用层粘连蛋白和Ⅳ型胶原的单克隆抗体对中度至重度成人慢性牙周炎龈上皮基底膜进行免疫组化研究，并结合电镜和ＰＡＳ技术加以分析。结果发现，正常时龈上皮基底膜呈连续均匀的带状分布，而炎症时则失去正常外观，出现多个散在的微小断裂，甚至个别上皮钉突基底膜缺失。这些改变在成人牙周类的发病中具有重要作用 Immunohistochemistry was performed using monoclonal antibodies to laminin and type IV collagen on the basement membrane of the epidermis of patients with moderate to severe chronic periodontitis. Electron microscopy and PAS were used to analyze the results. The results showed that the normal period of the gingival epithelial basement membrane was continuous and uniform ribbon distribution, but lost the normal appearance of inflammation, the emergence of multiple scattered tiny rupture, and even individual epithelial nail basement membrane missing. These changes play an important role in the pathogenesis of adult periodontal disease