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目的 了解广州汉族人在vWA31A等6个短串联重复序列(shorttandemrepeat,STR)位点法医学的有关数据。方法 运用STRPCR、4%变性聚丙烯酰胺凝胶电泳,结合荧光DNA自动检测技术,对汉族人群6个STR位点的等位基因频率和基因型频率进行了调查,并与其他种族或人群的等位基因频率进行了比较。结果 6个STR位点是vWA31A、TH01、F13A01、FES、TPOX和CSF1PO,所有位点的结果均符合HardyWeinberg平衡,且各位点等位基因间无相关性,除vWA31A外,其它5个位点的杂合度均低于白种人和黑种人。6个位点的累积DP=099999,PE=09708,pM=1059×105。结论 所得到的等位基因频率等数据可为中国汉族人群法医个人识别、亲子鉴定及遗传学研究提供依据。
Objective To understand the forensic data of 6 short tandem repeats (STR) loci such as vWA31A in Guangzhou Han population. Methods STR STR, 4% denaturing polyacrylamide gel electrophoresis combined with fluorescent DNA auto-detection technique were used to investigate the allele frequency and genotype frequency of 6 STR loci in Han population and to compare with other ethnic groups or population The gene frequencies were compared. Results Six STR loci were vWA31A, TH01, F13A01, FES, TPOX and CSF1PO. The results of all loci were in accordance with HardyWeinberg equilibrium, and there was no correlation between alleles at all loci. The other five loci except vWA31A Heterozygosity was lower than Caucasians and Blacks. The accumulation of 6 sites DP = 099999, PE = 09708, pM = 1059 × 105. Conclusion The obtained data of allele frequency and so on may provide the basis for the forensic personal identification, paternity test and genetics research of Chinese Han population.