论文部分内容阅读
目的:建立枳壳炮制前后的高效液相色谱指纹图谱,比较枳壳的药典炮制方法与江西地方炮制方法的不同。方法:采用高效液相色谱试验条件:C18色谱柱,甲醇∶水为流动相梯度洗脱,996PDA检测,分离分析枳壳炮制前后的醇提物和氯仿提取物的HPLC指纹图谱的变化。结果:枳壳生品与炮制品的醇提物在UVλ283 nm处所得特征峰几乎无变化,药典法枳壳炮制品和江西樟帮法炮制品的氯仿提取物在UVλ320 nm处所得的14号峰与枳壳原药材和建昌帮炮制品有显著的区别。结论:枳壳的药典炮制法与江西地方的樟帮法在水溶性和脂溶性部分的HPLC指纹图谱相似、与江西建昌帮炮制法在氯仿提取物的14号峰有显著差异。脂溶性部分的HPLC-FP可以作为枳壳炮制前后的特征指纹图谱。
OBJECTIVE: To establish HPLC fingerprints of Citrus aurantium (Fructus Aurantii) before and after processing, and to compare the methods of pharmacopoeia preparation of Citrus aurantium with that of Jiangxi. Methods: HPLC fingerprints of alcohol extract and chloroform extract of Citrus aurantium were separated and analyzed by high performance liquid chromatography (HPLC): C18 column and methanol: water as mobile phases. Results: The results showed that there was almost no change in the characteristic peaks of the extracts of Fructus Aurantii and processed products at UV λ283 nm. The chloroform extract of the Fructus Aurantii cultivated by the pharmacopoeia and the chloroform extract of the caesarean extract of Jiangxi Zhangbian Method showed the peak 14 at UV λ320 nm And Citrus aurantium herbs and Jianchang help products have significant differences. Conclusion: The pharmacopoeia of Fructus Aurantii is similar to the HPLC fingerprinting of water-soluble and lipophilic fractions in Zhangjiabang of Jiangxi Province. There is a significant difference in the HPLC fingerprinting of the chloroform extract from Jiancao Gang in Jiangxi Province. Lipid-soluble part of the HPLC-FP can be used as the characteristic fingerprints of Citrus aurantium processed before and after.