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目的探讨外源性YKL-40基因转染对前列腺癌LNcap细胞增殖、侵袭、迁移及黏附活性的影响。方法 RT-PCR法检测前列腺癌细胞LNcap、PC-3、DU-145 YKL-40基因内源性表达情况;用pcDNA3.1-YKL-40质粒转染LNcap细胞,分别经MTT法、Boyden小室法及黏附试验检测转染前后细胞增殖、侵袭、迁移及黏附活性,并进行体外药敏试验。结果仅DU-145细胞可内源性表达YKL-40基因;pcDNA3.1-YKL-40转染的LNcap细胞在第2～5天的A490值均显著高于对照组(P<0.05),其侵袭(75.11±4.40)和迁移穿膜细胞数(133.00±5.07)及黏附率(107.57%)均显著高于对照组(P<0.05),对5-FU、顺铂和依托泊苷的IC50值分别为(31.15±0.43)、(4.15±0.13)和(55.22±0.57)μmol/L,均显著高于对照组(P<0.05)。结论 YKL-40基因能促进LNcap细胞增殖,提高细胞侵袭、迁移及黏附活性,并使其对5-FU、顺铂和依托泊苷具有一定的耐药性。
Objective To investigate the effects of exogenous YKL-40 gene transfection on the proliferation, invasion, migration and adhesion of prostate cancer LNcap cells. Methods The endogenous expression of LNcap, PC-3 and DU-145 YKL-40 in prostate cancer cells was detected by RT-PCR. The LNcap cells were transfected with pcDNA3.1-YKL-40 plasmid by MTT method, Boyden chamber method And adhesion test before and after transfection to detect cell proliferation, invasion, migration and adhesion activity, and in vitro susceptibility testing. Results The expression of YKL-40 gene was only expressed in DU-145 cells. The A490 value of LNcap cells transfected with pcDNA3.1-YKL-40 was significantly higher than that of the control group at day 2 to 5 (P <0.05) The IC50 value of 5-FU, cisplatin and etoposide were significantly higher than that of the control group (75.11 ± 4.40) and the number of transmembrane cells (133.00 ± 5.07) and adhesion rate (107.57% (31.15 ± 0.43), (4.15 ± 0.13) and (55.22 ± 0.57) μmol / L, respectively, which were significantly higher than those in the control group (P <0.05). Conclusion YKL-40 gene can promote the proliferation of LNcap cells, increase cell invasion, migration and adhesion activity, and make it resistant to 5-FU, cisplatin and etoposide.