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Objective:To investigate the effect of Delisheng Injection(得力生注射液 DLS),a Chinese medicinal compound,DLS combined with cis-platinum(DDP),an active agent used in lung cancer chemotherapy,on a human highly metastatic giant lung carcinoma cell line PGCL3.Methods:The suspended PGCL3 cells at10~5/mL cultured in 96-well tissue culture plates were divided into 4 groups:DLS treatment group(2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL),DDP treatment group(1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL),combined DLS with DDP treatment group(DLS:DDP 2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL)and a control group.The cytotoxicity of DLS with different concentrations(2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL)on PGCL3 cells was determined by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT) assay.Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay.Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.Results:Compared with the control group,DLS(2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL) could significantly decrease cell proliferation,adhesion,invasion and migration abilities(P<0.05).Cell adhesion,invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP(2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL) compared with DDP single-agent treatment(1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL,P<0.05),respectively.Conclusions:DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis.Our research provided a experimental basis about the treatment on highly metastatic lung caner.
Objective: To investigate the effect of Delisheng Injection (DLS), a Chinese medicinal compound, DLS combined with cis-platinum (DDP), an active agent used in lung cancer chemotherapy, on a human highly metastatic giant lung carcinoma cell line PGCL3.Methods: The suspended PGCL3 cells at 10-5 / mL cultured in 96-well tissue culture plates were divided into 4 groups: DLS treatment group (2 μL / mL, 5 μL / mL, 10 μL / DDP treatment group (1 μg / mL, 2 μg / mL, 5 μg / mL, 15 μg / mL) combined DLS with DDP treatment group (DLS 2 μL / mL: / mL: 2 μg / mL, 10 μL / mL: 5 μg / mL, 25 μL / mL: 15 μg / mL) and a control group.The cytotoxicity of DLS with different concentrations (2 μL / , 10 μL / mL, 25 μL / mL) on PGCL3 cells was determined by 3- (4,5) -dimethylthiahiazo (-z-y1) -3,5-di- phenytetrazoliumromide (MTT) assay. Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay. Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells. Results: Compared to the control group, DLS (2 μL / mL, 5 μL / mL, 10 μL / mL, 25 μL / invasion and migration abilities were significantly decreased after combination treatment of DLS: DDP (2 μL / mL: 1 μg / mL, 5 μL / mL: 2 μg / mL, 10 μL / mL: 5 μg / mL, 25 μL / mL: 15 μg / mL) compared with DDP single-agent treatment (1 μg / mL, 2 μg / mL, , respectively.Conclusions: DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis. Our research provides a experimental basis about the treatment on highly metastatic lung caner.