Pentachloronitrobenzene (PCNB) is an organochlorine fungicide that is mainly used in the prevention and control of diseases in crop seedlings.Microbial removal is used as a promising method for in-situ removal of many organic pesticides and pesticide residues.A short-term field experiment (1 year) was conducted to explore the potential role of a PCNB-degrading bacterial isolate,Cupriavidus sp.YNS-85,in the remediation of a PCNB-contaminated soil on which Panax notoginseng was grown.The following three treatments were used:i) control soil amended with wheat bran but without YNS-85,ii) soil with 0.15 kg m-2 of solid bacterial inoculum (A),and iii) soil with 0.30 kg m-2 of solid bacterial inoculum (B).The removal of soil PCNB during the microbial remediation was monitored using gas chromatography.Soil catalase and fluorescein diacetate (FDA) esterase activities were determined using spectrophotometry.In addition,cultivable bacteria,fungi,and actinomycetes were counted by plating serial dilutions,and the microbial biodiversity of the soil was analyzed using BIOLOG.After 1 year of in-situ remediation,the soil PCNB concentrations decreased significantly by 50.3％ and 74.2％ in treatments A and B,respectively,when compared with the uninoculated control.The soil catalase activity decreased in the presence of the bacterial isolate,the FDA esterase activity decreased in treatment A,but increased in treatment B.No significant changes in plant biomass,diversity of the soil microbial community,or physicochemical properties of the soil were observed between the control and inoculated groups (P ＜ 0.05).The results indicate that Cupriavidus sp.YNS-85 is a potential candidate for the remediation of PCNB-contaminated soils under P.notoginseng.