目的　探讨非手术治疗胆囊结石的有效方法。方法　取外科手术中胆囊结石 30枚 ,放入 30只健康新西兰家兔胆囊中 ,并分为Ⅰ、Ⅱ、Ⅲ组。Ⅰ组给予 30 %柠烯一辛酸甘油酯和 30 %二甲基亚枫序贯灌注 ,Ⅱ组和Ⅲ组分别将两药单独灌注 ,72h后取出结石 ,测量用药前后结石重量。结果　Ⅰ组效果最好 ,Ⅱ组次之 ,Ⅲ组最差。结论　认为序贯灌注治疗胆囊结石具有良好的效果 ,优于单独灌注。 Objective To explore an effective method of non-surgical treatment of gallstone. Methods 30 cases of gallbladder stones in surgery were placed in 30 healthy New Zealand rabbits gallbladder and divided into Ⅰ, Ⅱ, Ⅲ group. Group Ⅰ was given 30% limonene caprylate and 30% dimethyl salicylate sequential perfusion. Groups Ⅱ and Ⅲ were given two drugs respectively. After 72 hours, the stones were removed and the weight of stones was measured before and after treatment. Results The results of group Ⅰ were the best, group Ⅱ was the second, group Ⅲ was the worst. Conclusions that sequential perfusion treatment of gallstone has a good effect, superior to perfusion alone.