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目的探讨中国汉族人群维生素D受体(vitaminDreceptor,VDR)基因多态性是否与乙型肝炎病毒(hepatitisBvirus,HBV)感染结局相关联。方法以184例慢性乙肝患者和205名无症状HBV携带者为研究对象,收集外周血,提取基因组DNA,应用聚合酶链反应-限制性片段长度多态性(polymerasechainreaction-restrictionfragmentlengthpolymorphism,PCR-RFLP)方法,分析VDR基因第2外显子FokⅠ位点、第9外显子TaqⅠ位点的多态性分布。结果单因素分析结果显示FokⅠ位点FF基因型在慢性乙肝组的频率44.6%显著高于无症状HBV携带组的31.7%(P<0.05)。经多因素非条件Logistic回归分析调整混杂作用后,结果仍然显示FF基因型在慢性乙肝组与无症状HBV携带组之间的差异存在统计学意义(OR=1.95,P<0·05)。FokⅠ位点与TaqⅠ位点组成的FT单倍型在慢性乙肝组的分布频率显著高于无症状HBV携带组(OR=1.45,P<0.05),fT单倍型在慢性乙肝组的分布频率显著低于无症状HBV携带组(OR=0.72,P<0.05)。结论维生素D受体基因多态性可能影响HBV感染的遗传易感性。
Objective To investigate whether the polymorphism of vitamin D receptor (VDR) gene is associated with hepatitis B virus (HBV) infection in Chinese Han population. Methods A total of 184 patients with chronic hepatitis B and 205 asymptomatic HBV carriers were enrolled in this study. Peripheral blood was collected and genomic DNA was extracted. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) The polymorphism distribution of Fok I and exon 9 Taq I of exon 2 of VDR gene was analyzed. Results The univariate analysis showed that the FF genotype of Fok I site was significantly higher in chronic hepatitis B patients (44.6%) than in asymptomatic HBV carriers (31.7%, P <0.05). After adjustment for the confounding effect by multivariate non-conditional logistic regression analysis, the results still showed that there was a significant difference in FF genotype between chronic hepatitis B group and asymptomatic HBV carrier group (OR = 1.95, P <0.05). The frequencies of F haplotypes and TaqI loci in chronic hepatitis B group were significantly higher than those in asymptomatic HBV carriers (OR = 1.45, P <0.05). The distribution frequency of fT haplotype in chronic hepatitis B group was significantly Lower than asymptomatic HBV carriers (OR = 0.72, P <0.05). Conclusion The polymorphism of vitamin D receptor gene may affect the genetic susceptibility of HBV infection.