将乙型肝炎表面抗原基因插入具调控型启动子ＰＨ０５的乳酸克鲁维酵母表达载体中，构建完成质粒ｐＬＳ１，转化宿主菌ＫｌｕｙｖｅｒｏｍｙｃｅｓｌａｃｔｉｓＣＸＪ１７Ａ，ＥＬＩＳＡ结果表明，其表达水平受无机磷浓度的调控。为了进一步提高表达水平，我们将ｐＬＳ１中的乙型肝炎表面抗原表达单元插入带完整ｐＫＤ１序列的载体ｐＥ１，并将构建成的质粒ｐＬＳ２转化ＭＷ９８８Ｃ。在比较了ＣＸＪ７Ａ／ｐＬＳ１和ＭＷ９８８Ｃ／ｐＬＳ２后，我们发现ＭＷ９８８Ｃ／ｐＬＳ２的稳定性大大提高，表达量也增加４～８倍 The hepatitis B surface antigen gene was inserted into Kluyveromyces lactis expression vector with the regulatory promoter PH05 to construct the plasmid pLS1 and transformed into Kluyveromyces lactis CXJ1-7A. The ELISA results showed that the expression level was controlled by inorganic phosphorus concentration. In order to further improve the expression level, we inserted the hepatitis B surface antigen expression unit of pLS1 into the vector pE1 with the complete pKD1 sequence and transformed the constructed plasmid pLS2 into MW98-8C. After comparing the CXJ 7A / pLS1 and MW98 8C / pLS2, we found that MW98 8C / pLS2 greatly improved the stability, the expression also increased 4 to 8 times