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以苹果栽培品种嘎拉试管苗带柄叶片为材料,接种于附加不同浓度BA和蔗糖及不同氨基酸的MS培养基上,诱导叶柄产生体细胞胚和再生嫩梢。研究显示,附加BA 1.5mg/L的MS培养基是叶柄形成体细胞胚进而发育成嫩梢的适宜培养基。培养基中蔗糖浓度调控叶柄的分化类型,浓度在5～10g/L时,叶柄由体细胞胚途径分化嫩梢,浓度为20g/L时,叶柄同时由体细胞胚和器官发生途径形成嫩梢,浓度30g/L以上时,叶柄由器官发生途径分化嫩梢。4种氨基酸中,L-脯氨酸和谷氨酰胺促进叶柄体细胞胚形成,以L-脯氨酸促进效果最好。
Apple cultivars Gala plantlets with handle leaves were inoculated on MS medium supplemented with different concentrations of BA, sucrose and different amino acids to induce the production of somatic embryos and regenerate shoots in petioles. Studies have shown that supplemented BA 1.5mg / L of MS medium is petiole formation of somatic embryos, and then develop into shoots of the appropriate medium. In the culture medium, sucrose concentration regulated the type of petiole differentiation. When the concentration was 5 ~ 10g / L, the petiole was differentiated into new shoots by somatic embryogenesis. When the concentration was 20g / L, the petioles formed young shoots through somatic embryogenesis and organogenesis , The concentration of 30g / L or more, the petiole differentiation by the organogenesis shoots. Of the four kinds of amino acids, L-proline and glutamine promoted the formation of somatic embryo somatic embryos, and L-proline was the best.