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以花生成熟种胚为外植体 ,在 MS附加不同激素 ( 2 0 mg/ L 2 ,4 -D或 5mg/ L Picloram)的培养基上黑暗培养 ,胚性愈伤组织发生率和产胚量无明显差异。在 MS+5mg/ L Picloram培养基上 ,胚性愈伤组织发生率和产胚量与品种类型有关 ,珍珠豆型花生品种胚性愈伤组织发生率和产胚量高于普通型花生品种。体胚在MS+1 0 mg/ L BA培养基上小苗再生达 36.3%～ 77.8%。再生小苗在 MS+0 .3mg/ L NAA培养基上生根后 ,移栽温室能正常生长并开花、结荚
The mature embryo of peanut was used as explants. The darkness culture, embryogenic callus incidence and embryo yield in medium supplemented with different hormones (20 mg / L 2, 4-D or 5 mg / L Picloram) No significant difference. In MS + 5mg / L Picloram medium, the incidence of embryogenic callus and embryo yield were related to the type of cultivars. The embryogenic callus and peanut embryo yield of peanut pea cultivars were higher than that of common peanut cultivars. Somatic embryos regenerated from 36.3% to 77.8% in MS + 10 mg / L BA medium. Regeneration seedlings in MS +0. 3mg / L NAA medium after rooting, transplanting greenhouse can grow normally and flowering, podding