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目的:研究拟以MHV-A59和大肠杆菌作为生物致病因子,建立两种小鼠温病湿热证动物模型,通过对比探讨小鼠肝炎病毒温病湿热证模型可行性。方法:动物随机分为3组:正常组、模型组(肥甘饮食+湿热外环境+MHV-A59造模)、肠菌组(肥甘饮食+湿热外环境+大肠杆菌造模),检测小鼠血清TG、TNF-α水平及肝组织病理切片,观察动物症状及体征。结果:与正常组比较,两种温病湿热证模型组小鼠血清TG及TNF-α水平明显升高(P<0.01),两组造模动物均不同程度出现了发热、疲倦、纳呆、腹泻、体重减少、毛发无泽等症状。结论:以MHV-A59作为生物致病因子,结合肥甘饮食及湿热外环境因素构建的动物模型,可以作为温病湿热证动物模型之一,也是一种新的病证结合模型。
OBJECTIVE: To establish an animal model of damp-heat syndrome in mice with both MHV-A59 and Escherichia coli as bio-pathogenic factors, and to compare the feasibility of the mouse model of warm-damp-heat syndrome with hepatitis virus. Methods: The animals were randomly divided into 3 groups: normal group, model group (Feigan diet + humid environment + MHV-A59 modeling), enterobacteriaceae (Feigan diet + damp-heat environment + E.coli modeling) Serum TG, TNF-α levels and liver biopsy were observed animal symptoms and signs. Results: Compared with the normal group, the levels of serum TG and TNF-α in the two warm-dampness-heat syndrome model groups were significantly increased (P <0.01). The animals in both groups showed fever, tiredness, Diarrhea, weight loss, no hair and other symptoms Ze. Conclusion: Animal model with MHV-A59 as bio-pathogenic factor combined with fat diet and external factors of dampness and heat can be used as one of the animal models of damp-heat syndrome of warm-blooded disease and a new syndrome-disease-syndrome model.