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利用3对AFLP引物对我国棉褐带卷蛾Adoxophyes orana(Fischer von Rosslerstamm)4个地理种群进行了遗传分化研究。共扩增得到224个DNA条带,其中多态条带195个;遗传多样性分析表明各群体均具有较高的遗传多样性水平,Nei遗传多样性指数为0.1425~0.1578,Shannon多样性指数为0.2137~0.2383。群体间的遗传分化系数(Gst)和基因流(Nm)分析结果表明,北方的苹小卷叶蛾与南方茶小卷叶蛾之间产生了明显的遗传分化,而北方的苹小卷叶蛾不同种群间则存在着大量的基因交流,遗传分化程度很弱。AMOVA分析结果显示66.81%的变异来源于种群内。UMAGA聚类分析表明,北京种群、陕西种群和山东种群间遗传距离较小,聚在一起为一分支,福建的茶小卷蛾单独为一分支。推测棉褐带卷蛾种群间的遗传分化与寄主有关联,与地理距离不相关。
Three pairs of AFLP primers were used to study the genetic differentiation of four geographic populations of Adoxophyes orana (Fischer von Rosslerstamm) in China. A total of 224 DNA bands were amplified, of which 195 were polymorphic bands. Genetic diversity analysis showed that each population had a high level of genetic diversity, Nei’s genetic diversity index was 0.1425 ~ 0.1578, Shannon’s diversity index was 0.2137 ~ 0.2383. The genetic differentiation coefficient (Gst) and gene flow (Nm) analysis showed that there was a clear genetic differentiation between N. dorsalis and S. dorsalis in the north, and between different populations in the north A large number of gene exchange, genetic differentiation is weak. AMOVA analysis showed that 66.81% of the variation came from within the population. UMAGA cluster analysis showed that the genetic distance between Beijing population, Shaanxi population and Shandong population was small and clustered together as a branch. It is speculated that the genetic differentiation among populations of cotton bollworm is related to the host and not related to the geographical distance.