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目的:研究臭牡丹总黄酮体外对培养的人肝癌Hep G2细胞增殖的影响。方法:提取中药臭牡丹的有效成分臭牡丹总黄酮,采用不同浓度分别作用于培养的Hep G2细胞,通过细胞形态学和MTT法检测臭牡丹总黄酮对Hep G2细胞增殖的影响。结果:臭牡丹总黄酮药物浓度在0.025、0.25、2.5、25、250μg/m L时的抑制率分别为5.55%、12.73%、14.84%、62.44%、76.81%,与对照组比较差异均有统计学意义(P<0.05)。结论臭牡丹总黄酮体外对人肝癌Hep G2细胞的增殖有明显抑制作用。
Objective: To study the effect of total flavonoids of Peony on the proliferation of Hep G2 cells cultured in vitro. Methods: Total flavonoids of Peony stolonifer were extracted from stinky peony. The effects of flavonoids from stinky peony on proliferation of Hep G2 cells were detected by MTT assay and cell morphology respectively. Results: The inhibitory rates of total flavonoids of Stinky Peony at the concentrations of 0.025, 0.25, 2.5, 25 and 250 μg / m L were 5.55%, 12.73%, 14.84%, 62.44% and 76.81%, respectively Significance (P <0.05). Conclusion The total flavonoids of Peony had a significant inhibitory effect on the proliferation of Hep G2 cells in vitro.