论文部分内容阅读
目的:优选姜椒凝胶剂的处方工艺并探讨其体外经皮渗透性。方法:以辣椒素24 h单位面积累积渗透量为评价指标,通过L9(34)正交试验考察甘油、丙二醇、氮酮用量对姜椒凝胶处方工艺的影响;以大鼠离体皮肤为屏障,运用Franz扩散池作为体外经皮释药模型,考察姜椒凝胶的体外透皮特性。采用HPLC测定辣椒素含量,色谱条件为Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%磷酸水(65∶35),流速1.0 mL·min-1,检测波长280 nm,柱温40℃,进样量20μL。结果:姜椒凝胶处方的最佳组合为2%卡波姆,10%甘油,20%丙二醇和1%氮酮;其体外经皮渗透符合零级动力学方程,经皮渗透速率0.353μg·h-1·cm-2。结论:该处方设计合理,适宜皮肤局部用药。
Objective: To optimize the prescription of ginger gel and to explore its in vitro permeability. Methods: The cumulative permeation amount of capsaicin 24 h per unit area was taken as the evaluation index. The effects of the dosage of glycerol, propylene glycol and azone on the prescription of ginger gel were investigated by L9 (34) orthogonal test. , The Franz diffusion cell was used as in vitro transdermal delivery model to investigate the in vitro transdermal properties of ginger pepper gel. The content of capsaicin was determined by HPLC. The chromatographic conditions were Diamonsil C18 column (4.6 mm × 250 mm, 5 μm), mobile phase methanol-0.1% phosphoric acid water (65:35), flow rate 1.0 mL · min- , Column temperature 40 ℃, injection volume 20μL. Results: The optimal combination of ginger gel gel prescription was 2% carbomer, 10% glycerol, 20% propylene glycol and 1% azone. The transdermal permeation in vitro was in accordance with the zero order kinetic equation and the transdermal permeation rate was 0.353 μg · h-1 · cm-2. Conclusion: The prescription design is reasonable, suitable for topical skin application.