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微核率是检测各种理化因子诱发染色体断裂损伤或影响细胞正常分裂中染色体行为的常用实验方法。由于实验条件的限制,生物因子对微核率影响的研究报道很少。本实验采用遗传背景和生活条件均相同的无菌大鼠和普通大鼠做对比实验(无菌大鼠由本所悉生生物室培育),以探求生物因子对骨髓嗜多染红细胞微核率的影响和无菌动物用于检测环境致突变理化因子的可能。实验分4组:(1)无菌大鼠10只;(2)无菌大鼠—丝裂霉素C(Japan)6只;(3)普通大鼠7只;(4)普通大鼠—丝裂霉素C6只。丝裂霉素C以4mg/kg腹腔注射,24小时后取骨髓细胞,用大鼠血清混匀,涂片,Giemsa染色取计数微核率:(1)无菌大鼠3/10000;(2)无菌大
Micronucleus rate is a commonly used experimental method to detect chromosomal breakage induced by various physicochemical factors or to affect chromosome behavior in normal cell division. Due to the limitation of experimental conditions, there are few reports on the effects of biological factors on the micronucleus rate. In this experiment, sterile rats and normal rats with the same genetic background and living conditions were used for comparison experiments (sterile rats were cultured in the biological chambers of this study) in order to search for the micronuclear rate of bone marrow polychromatic erythrocytes by biological factors. Affected and sterile animals are used for the detection of environmental mutagenic physicochemical factors. The experiment was divided into 4 groups: (1) 10 sterile rats; (2) 6 sterile rats - Mitomycin C (Japan); (3) 7 normal rats; (4) normal rats - Mitomycin C6 only. Mitomycin C was intraperitoneally injected at 4 mg/kg. After 24 hours, bone marrow cells were collected, mixed with rat serum, and smeared. The micronucleus rate was counted by Giemsa staining: (1) sterile rat 3/10000; (2 Aseptic