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目的探讨线粒体分裂-动力相关蛋白1(Drp1)及其抑制剂Mdivi-1在醛固酮肾损伤小鼠足细胞中的作用及可能机制。方法 20只小鼠随机均分为假手术组(A组)、Mdivi-1处理组(B组)、醛固酮损伤组(C组)和醛固酮损伤+Mdivi-1处理组(D组)。采用Western blot检测Drp1、细胞色素C(Cyt C)及足细胞裂孔隔膜蛋白nephrin、podocin的表达,免疫荧光技术检测肾小球足细胞中Drp1定位,酶标仪检测半胱天冬氨酸蛋白酶3(Caspase-3)、Caspase-9的活性。结果与A组相比,C组Drp1表达及Caspase-3、Caspase-9活性增加,Cyt C向细胞质释放增多,nephrin、podocin表达降低(P<0.01);而D组Mdivi-1处理后能逆转C组各观察指标的变化过程(P<0.01)。结论 Drp1可能通过线粒体凋亡途径减轻醛固酮诱导的足细胞损伤。
Objective To investigate the role and possible mechanism of mitochondrial split-dynamic-related protein 1 (Drp1) and its inhibitor Mdivi-1 in podocytes of aldosterone-induced renal injury in mice. Methods Twenty mice were randomly divided into sham operation group (A group), Mdivi-1 treatment group (B group), aldosterone injury group (C group) and aldosterone injury + Mdivi-1 treatment group (D group). Western blot was used to detect the expressions of Drp1, Cyt C, podocin and podocin in the podocytes. Immunofluorescence staining was used to detect the localization of Drp1 in glomerular podocytes. The level of caspase 3 (Caspase-3), Caspase-9 activity. Results Compared with group A, the expressions of Drp1, Caspase-3 and Caspase-9 in group C were increased, the release of Cyt C in the cytoplasm was increased and the expressions of nephrin and podocin were decreased in group C (P <0.01) The changes of each observation index in group C (P <0.01). Conclusion Drp1 may reduce aldosterone-induced podocyte injury through the mitochondrial apoptotic pathway.