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目的探究苯并[α]芘(B[α]P)对海马学习记忆能力的毒性作用及丁基羟基茴香醚(BHA)对此的保护作用。方法 90只雄性SD大鼠随机分为空白对照组、溶剂对照组、B[α]P处理组〔(2mg/(kg·d)〕、BHA处理组〔50mg/(kg·d)〕和B[α]P+BHA联合处理组。按分组及大鼠体质量给予相应剂量灌胃处理(空白对照组、溶剂对照组用等量生理盐水、花生油处理),1次/d,持续90d。暴露90d后采用Morris水迷宫测试大鼠学习记忆能力;处死大鼠,剥离脑组织,检测海马组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)、ATP酶活性及海马突触体内Ca2+浓度。结果 Morris水迷宫行为学测试结果显示,与其他组相比,B[α]P组逃避潜伏期增加,跨平台次数减少,差异均有统计学意义(P<0.05);海马中MDA〔(2.46±0.39)nmol/mg prot.〕含量及Ca2+浓度〔(146.3±16.68)nmol/L〕升高,而SOD〔(76.1±11.42)nmol/mg prot.〕和ATP酶活性降低(P均<0.05)。B[α]P+BHA联合处理组较B[α]P组各指标改善(P均<0.05),且与溶剂对照组比较,差异无统计学意义。结论 B[α]P引起的神经行为毒性作用可能与海马ATP酶活性降低及Ca2+浓度增加等氧化损伤有关,而BHA可防止此类损伤。
Objective To explore the toxic effects of benzo [α] pyrene (B [α] P) on the learning and memory abilities of hippocampus and the protective effects of butylated hydroxyanisole (BHA). Methods Ninety male Sprague-Dawley rats were randomly divided into control group, B [α] P treatment group (2mg / (kg · d)], BHA treatment group [50mg / (kg · d) [α] P + BHA combined treatment groups were given the corresponding dose gavage and body weight control group (blank control group, solvent control group with the same amount of saline, peanut oil treatment), 1 times / d, continued for 90 days. After 90 days, Morris water maze test was used to evaluate the learning and memory ability of rats. Rats were sacrificed and the brain tissue was peeled off. The contents of malondialdehyde (MDA), superoxide dismutase (SOD), ATPase and hippocampal synaptosomal Ca2 + Results The Morris water maze test showed that compared with other groups, the escape latency and the number of cross-platform decreased in B [α] P group (P <0.05) (2.46 ± 0.39) nmol / mg prot.] And Ca2 + concentration 〔(146.3 ± 16.68) nmol / L〕, while the SOD 〔(76.1 ± 11.42) nmol / mg prot.〕 And ATPase activity decreased (P < 0.05) .At the same time, B [α] P + BHA combined treatment group improved compared with B [α] P group (P <0.05), and there was no significant difference compared with solvent control group.Conclusion B [α] God Behavioral toxicity may be reduced hippocampus ATP activity and Ca2 + concentration increased oxidative damage, whereas BHA prevents such damage.