对１８例结直肠癌患者采用实体瘤短期体外悬浮细胞培养法进行染色体分析。结果：染色体结构重排涉及１７ｑ、５ｑ、６ｑ（７８％、６１％、５０％），染色体增加频率最高的是７号、３号（７２％、６６％），丢失则最常见于１７号、５号、１８号（５０％、４４％、３３％）。大量标记染色体、双微体及超倍体核型预示肿瘤易早期复发和转移。此结果提示：１７号、５号及１８号染色体畸变与结直肠癌发生发展有密切关系。非随机性染色体异常的多样性和复杂性更表明大肠癌的发生是多基因多步骤演变的结果。 In 18 patients with colorectal cancer, chromosome analysis was performed using solid tumors in vitro suspension cell culture method. Results: Chromosome rearrangements involved 17q, 5q and 6q (78%, 61%, 50%). Chromosome increased frequency was the highest on chromosomes 7 and 3 (72%, 66%), and loss was most common on the 17th. No. 5 and No. 18 (50%, 44%, 33%). A large number of markers of chromosomes, double-microsomes, and hyperploid karyotypes are predictive of early recurrence and metastasis of tumors. This result suggests that the chromosome 17th, 5th, and 18th aberrations are closely related to the occurrence and development of colorectal cancer. The diversity and complexity of non-random chromosomal abnormalities further indicate that the occurrence of colorectal cancer is the result of multi-step multi-gene evolution.