目的 优选人脑胶质瘤细胞诱导分化剂。方法 应用MTT法、形态学、GFAP免疫荧光等方法鉴定环已亚甲基二乙酰胺、二甲基甲酰胺、丁酸钠、二丁酰杯AMP、苯乙酸钠、苯丁酸钠、亚砷酸钠等7种诱导剂对人脑胶质瘤细胞系SHG-44的诱导分化效果。结果 亚砷酸钠、苯乙酸钠抑制肿瘤细胞增殖效果较差,苯丁酸钠、二丁酰环AMP、丁酸钠在1-2mM即可明显诱导人脑胶质瘤细胞多分化,表现为增殖抑制,胞突增长,GFAP表达升高现象。结论 人脑胶质瘤细胞对诱导分化剂苯丁酸钠、二丁酰环AMP和丁酸钠较为敏感,值得深入研究。 Objective To optimize differentiation of human glioma cells. Methods MTT, morphological and GFAP immunofluorescence were used to identify the effects of cyclodextrin, dimethylformamide, sodium butyrate, dibutyryl AMP, sodium phenylacetate, sodium phenylbutyrate, Induced differentiation of human glioma cell line SHG-44 by using seven kinds of inducer such as sodium and sodium. Results Sodium arsenite and sodium phenylacetate had a poorer effect on inhibiting the proliferation of tumor cells. Sodium phenylbutyrate, dibutyryl cyclic AMP and sodium butyrate could obviously induce the multi-differentiation of human glioma cells at 1-2mM, , Cell growth, GFAP expression increased. Conclusion Human glioma cells are sensitive to the inducer of sodium phenylbutyrate, dibutyryl cyclic AMP and sodium butyrate, which deserves further study.