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实验观察了多种浓度的梭曼对大鼠离体海马脑片CA 1区锥体层诱发场电位的影响。结果如下:(1)梭曼可迅速而显著地抑制顺向诱发场EPSP和群体锌电位,使其振幅、面积减小,潜伏时延长。这种抑制效应具有剂量依赖性。撤除梭曼后,被抑制的电位可以不同程度的自动恢复。恢复后,再次应用梭曼,可重复出现同样的抑制效应。(2)梭曼可迅速地使逆向诱发群体锋电位幅度增大,以灌流梭曼溶液的最初20分钟内为显著。(3)在33例脑片上,不论是顺向或逆向刺激,所用各种浓度梭曼均未能诱发CA 1区锥体细胞产生癫痫样电活动。而用青霉素则能诱发该细胞群体产生痫癫样放电。结果提示,梭曼对海马脑片的突触传递过程具有抑制作用,而对海马脑片CA 1区锥体细胞似无直接的致痫作用。
The effects of various concentrations of soman on evoked field potential of CA 1 pyramidal neurons in rat hippocampal slices were observed. The results are as follows: (1) Soman can rapidly and significantly inhibit the EPSP and zinc potential in the induced field to decrease amplitude and area and prolong latent period. This inhibitory effect is dose-dependent. After the removal of soman, the suppressed potential can be automatically restored to varying degrees. After recovery, the same inhibitory effect can be repeated with soman again. (2) Soman can rapidly increase the amplitude of the reverse evoked population spikes to be significant within the first 20 minutes of perfusion of the soman solution. (3) In all 33 brain slices, all concentrations of soman used in either CA1 area or CA1 area failed to induce epileptiform activity in CA 1 pyramidal cells. While using penicillin can induce epileptiform epileptiform discharges in this cell population. The results suggest that soman inhibits the synaptic transmission in hippocampal slices, while there is no direct epileptogenic effect on pyramidal cells in CA 1 area of hippocampal slices.