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构建携带人IL-10和EGFP双顺反子的腺病毒载体,检测其对原代培养的豚鼠肝细胞体外转染的效力和h IL-10的体外表达水平,为下一步的肝脏基因治疗实验做准备。1材料与方法1.1材料质粒载体pUC 19、pDC 315、含有脑心肌炎病毒IRES的质粒pCLON 13、腺病毒载体pBHGE 3及腺病毒E 1区转化
To construct the adenoviral vector carrying human IL-10 and EGFP bicistronic, to test the in vitro transfection efficiency of primary cultured guinea pig hepatocytes and the expression level of hIL-10 in vitro, which is the next step of liver gene therapy experiment prepare. 1 Materials and Methods 1.1 Materials Plasmid vectors pUC 19, pDC 315, plasmid pCLON 13 containing the encephalomyocarditis virus IRES, the adenoviral vector pBHGE 3 and the adenovirus E 1 region were transformed